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KMID : 0357820120360010074
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Korean Journal of Legal Medicine
2012 Volume.36 No. 1 p.74 ~ p.84
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DNA- Based Identification of Necrophagous Fly Species Using Abdominal-B (Abd-B) Homeobox Sequence
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Piao Hu-Guo
Chung Uk-Hee
Shin Shang-Eon
Ko Kwang-Soo
Hwang Juck-Joon
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Abstract
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In medicolegal investigations, correct identification of the necrophagous fly species collected around and on the corpse is an essential step for estimating the postmortem interval (PMI). Therefore, forensic pathologists and entomologists investigating deaths due to violent crimes need a rapid, easy-to-use protocol to identify fly species found on corpses. A rapid and robust DNA-based tool that can distinguish between various immature and mature species from the Calliphoridae, Muscidae, and Sarcophagidae families would be ideal for such investigations. To date, the DNA barcode initiative is the best approach for identifying species-specific nucleotide sequences. We have developed 3 sequence-characterized amplified region (SCAR)-based identification systems derived from the Abdominal-B homeobox sequences of 17 fly species belonging to the Muscidae and Sarcophagidae. The flies used in this study were collected in Korea. These assay systems can classify 17 forensically important flyspecies into the dipteran family group and reliably distinguish them from inter- and intraspecific fly species through a 2-step multiplex PCR. This novel approach may also be used as an alternative to conventional DNA-based identification methods.
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KEYWORD
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necrophagous fly, Abdominal-B, identification, postmortem interval
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